This means that it can finely … If you click accept cookies then all cookies will be written. BD Accuri C6 Plus flow cytometer. 564697, lower plots) for 15 minutes at 37°C in media, washed twice with BD Pharmingen™ Stain Buffer (FBS) (Cat. Students perform cell staining with an antibody panel designed to differentiate various white blood cell (WBC) populations (see figure). Until 2001, platelets were commonly counted manually using a hemocytometer or automated impedance counter, despite the serious limitations of these methods. All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. Samples were acquired on a BD Accuri C6 in the 2-blue, 2-red configuration using the BD CBA Flex Set Accuri template and analyzed using FCAP Array software v3.0.1. All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri C6 flow cytometer systems. During manufacture, laser and optical alignments are set and locked down. Finally, it can measure several cytokines simultaneously for an individual cell. All rights reserved. Microalgae can vary greatly in size, can live as single cells or in colonies, and cultures grown in open ponds often contain debris and other contaminating organisms. No. Free BD Accuri software templates simplify data collection and analysis. The white paper presents sample data and guidance for viable cell concentrations in cultured cell lines, immune cell concentrations in human peripheral blood, and platelet counts in whole, unlysed human blood. The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. After harvesting and staining the cells with 7-AAD and either FITC or APC anti-BrdU according to the kit protocol, samples were acquired on a BD Accuri C6 flow cytometer using the kit template, and analyzed using BD Accuri C6 software. All current service contracts will be honored. BD Accuri systems also interface smoothly with automated bioreactor sampling systems so that samples can be taken continuously and analyzed automatically. The BD Accuri C6 is a compact flow cytometer that uses a low-pressure pumping system to drive the fluidics allowing for the derivation of sample volume and calculation of absolute counts or sample concentration per microliter. No. Not for use in diagnostic or therapeutic procedures. Distinguishing DNA content and ploidy in A. thaliana root tissues. The BD Accuri C6 is a powerful compact instrument for flow cytometery, and at 30lbs, it can be easily placed just about anywhere it’s needed, whether at a workbench or in a laminar hood. The experiment was repeated under three different temperature conditions: at room temperature, in a 37°C water bath, and on ice. Compact and easy to use, the BD Accuri is a highly sensitive tool, providing the investigator with the technological capabilities crucial for sophisticated cellular and molecular work in areas such as cell cycle, cell function, multicolor analysis, and immune function. This feature speeds and simplifies cell analysis by calculating cell concentrations (per unit sample volume) automatically. After fluorescence gating, light scatter can distinguish bacteria and yeast, Determination of bacterial strain by PNA FISH. However, plant nuclear DNA content (ploidy) varies over extreme ranges, and nuclear DNA measurements of plants are often hampered by excessive cellular and subcellular debris and autofluorescence from other prevalent cellular components, such as chloroplasts. To add test compounds to the cell suspension, a “stop-flow” method is often used in which sampling is paused, the sample tube opened, the agonist added, and the tube resealed. 560082). Accuri C6 is a digital PC based bench top analyser, 2 laser system. In some cases, this information might be enough to identify specific microorganisms in a heterogeneous sample. Many research and teaching institutions introduce graduate students to flow cytometry as part of their training and research. BD Accuri (Becton Dickinson, San Jose, CA, USA) Data File Structure: BD Accuri C6 Software files (.c6) Software: BD Accuri C6 software Laser: Blue laser 488nm Red laser 640nm Fluorescence detection: 3 wavelengths detected from 488-nm laser: 533/30 nm 585/40 nm This option significantly expands the fluorochrome combinations you can analyze. The BD™ Cell Viability Kit provides a simple, two-color method to monitor microbial cell viability on BD Accuri flow cytometers. With its compact 11 x 14.75 x 16.5-inch footprint, light weight of 30 lb, and operational simplicity, the BD Accuri C6 Plus supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. Software options and instrument controls are clearly visible from the software’s tabbed interface. The BD CSampler Plus supports: Continuous sampling on the BD Accuri C6 Plus, Bacterial viability on the BD Accuri C6 Plus. Accuri™ C6 Becton Dickinson. Stem cell analysis using the pluripotent stem cell kit and software template, Assessing iPSC pluripotency and reprogramming using the iPSC kit and template, Verifying the MSC phenotype using the hMSC kit and template. 10 x 75 µm . ), Human PBMCs were stained with the BD Pharmingen Human Regulatory T-Cell Cocktail (Cat. With Accuri's new Selectable Lasers Module, the numerous fluorophore combinations that the C6 … Apoptotic cells can be detected alone or simultaneously along with DNA damage and cell proliferation. Each group received a different compound or compound concentration and added it to the culture. So the choice should be addressed by the kind of flow experiments you plan to do. Some authorities argue that algal biofuel is the best renewable candidate to fully replace fossil petroleum. Nos. 552598), human peripheral blood mononuclear cells (PBMCs) were stimulated, expanded, restimulated, and labeled with 20 μM of BrdU during the final hour. Accuri C6 is a digital PC based bench top analyser, 2 laser system. Flow cytometry is a quick and reliable methodology for identifying these populations simultaneously in a single sample, based on their immunophenotypes (see table). ACCURI C6 Tweeter ... NOUVEAU module de changement de configuration des lasers: voir les différentes configurations possibles (et la liste des fluorochromes associés) Pas de réglages de PMT nécessaire! Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. La vitesse de flux maximum est de 10 000 évènements par seconde. During manufacture, laser and optical alignments are set and locked down. In addition, C6 has a flow metering system for automatic calculation of absolute cell counts. BD T-cell cytokine kits and reagents simplify the detection of cytokines using intracellular flow cytometry on BD Accuri flow cytometers. Side scatter and CD14 expression were used to discriminate lymphocyte and monocyte populations. of … Click the link below to read about the impactful work done by the small champion, BD Accuri™. Flow cytometry can rapidly and accurately immunophenotype many kinds of cells, including lymphoid tissue (thymus, spleen, and lymph nodes), digested solid tissues, and blood. Fluidics are cleaned automatically on instrument shutdown, and laboratory-grade water is used as sheath fluid, reducing operating costs. Its adaptability and suitability for experiments up to four colors have allowed researchers to publish numerous scientific articles citing BD Accuri flow cytometers. Student groups at California State University, Northridge, cultured CEM-C7-14 leukemia cells at a designated cell density. The kits include or specify transport inhibitors, buffer systems, and fluorescent antibodies needed for acquisition and analysis. SYBR® is a registered trademark of Life Technologies Corporation. Find out how this powerful platform, combined with BD kits and templates, will give you more insight and easier workflow. BD Accuri™ flow cytometers offer particular advantages for industrial and agricultural applications. Assessing Th1, Th2, and Th17 cytokines using the phenotyping kit and template, Assessing cytotoxic T-cell cytokines using the kit and template, Cytokine analysis by analyte or sample using BD CBA kits. No. You can analyze cell cycle and aneuploidy or trace signaling cascades across multiple subpopulations. Using lipophilic dyes, BD Accuri systems can rapidly estimate neutral lipid storage in small samples of live algal cells, usually without needing to remove the alga from its growth medium. Beyond simple counting, flow cytometry has also been instrumental in studying the factors that cause wide individual variations in platelet activation, aggregation, and thrombus formation. To streamline sample processing, the BD CSampler allows multiple collection settings to be applied to plate or tube runs. Despite its compact size, the optical system demonstrates extraordinary fluorescence sensitivity. Table: Accurate event counts per mL of water sample can then be determined using BD Accuri C6 software. detector(s). All tasks and settings are laid out simply on one screen for quick access and operation. The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. Available to trained users with online booking. The BD Accuri™ is proof that small can not only be efficient but can also make a big impact. All samples were prepared using the BD™ CBA Human Soluble Protein Master Buffer Kit (Cat. Eye and skin exposure to direct and reflected laser light is hazardous and may be extremely harmful. Software: Accuri C6 Software and CSample Software. Human whole blood was drawn into heparinized tubes and stimulated with PMA (10 ng/mL) and Ionomycin (1 µg/mL) for 5 hours at 37°C in the presence of 10 ng/mL of Brefeldin A (BD GolgiPlug, Cat. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. Data was generated on either the BD Accuri C6 Plus or the BD Accuri C6 as indicated in figure legends. Laser Power . C6 Configuration: The C6 contains two collinear lasers and is arranged in such a way that four detectors all … Flow cytometry can rapidly quantitate bacteria and discriminate them from debris. Researchers can use intracellular flow cytometry to assess production of key cytokines at the single-cell level. BD Accuri systems can help you quickly examine reporter gene transfection efficiency and cell population heterogeneity—right from your benchtop. Direct counts on BD Accuri systems correlate highly with counting beads, and are more precise than hemocytometer counts. No. The cells were then fixed, lysed and permeabilized using the BD Pharmingen™ Human Transcription Factor Buffer Set (Cat. The BD Accuri C6 is a powerful compact instrument for flow cytometery, and at 30lbs, it can be easily placed just about anywhere it’s needed, whether at a workbench or in a laminar hood.

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